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Measurement of gum and serum 8 OHdGFrom the homogenized gingival samples, MtDNA was isolated from rat gingiva using a DNA financial institution kit(Wako Pure Chemical sectors, Osaka, Okazaki, japan)13. Isolated mtDNA and serum were analyzed by a cheap enzyme linked immunosorbent assay method using a"High critical 8 OHdG Check" Product(Japan Institute for the charge of Aging, Shizuoka, The japanese).
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